Fluorinated substrates result in variable leakage of a reaction intermediate during catalysis by dehydroquinate synthase.
نویسندگان
چکیده
Incubation of (3S)-3-fluoro-3-deoxy-D-arabino-heptulosonate 7-phosphate with dehydroquinate (DHQ) synthase from three phylogenetically distinct sources resulted in the production of (6S)-6-fluoroDHQ and its epimer 1-epi-(6S)-6-fluoroDHQ. The differences in the product ratios of the reactions catalysed by each enzyme imply that 1-epi-(6S)-6-fluoroDHQ formation occurs by an unusual partial leakage of a reaction intermediate from the enzyme.
منابع مشابه
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عنوان ژورنال:
- Organic & biomolecular chemistry
دوره 9 8 شماره
صفحات -
تاریخ انتشار 2011